Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer
نویسندگان
چکیده
منابع مشابه
Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer
INTRODUCTION The present study compares bone morphogenetic protein (BMP)-4 and BMP-2 gene transfer as agents of chondrogenesis and hypertrophy in human primary mesenchymal stem cells (MSCs) maintained as pellet cultures. METHODS Adenoviral vectors carrying cDNA encoding human BMP-4 (Ad.BMP-4) were constructed by cre-lox combination and compared to previously generated adenoviral vectors for B...
متن کاملCilomilast enhances osteoblast differentiation of mesenchymal stem cells and bone formation induced by bone morphogenetic protein 2.
A rapid and efficient method to stimulate bone regeneration would be useful in orthopaedic stem cell therapies. Rolipram is an inhibitor of phosphodiesterase 4 (PDE4), which mediates cyclic adenosine monophosphate (cAMP) degradation. Systemic injection of rolipram enhances osteogenesis induced by bone morphogenetic protein 2 (BMP-2) in mice. However, there is little data on the precise mechanis...
متن کاملRole of bone morphogenetic protein-2 in osteogenic differentiation of mesenchymal stem cells
Bone mesenchymal stem cells (BMSCs) have been an area of interest in biomedical research and tissue engineering due to their diverse differentiation abilities. In osteogenesis, bone morphogenetic proteins (BMPs), particularly BMP‑2, are important. However, the effect of BMP‑2 on the osteogenetic capacity of BMSCs remains to be fully elucidated. In the present study, primary rat BMSCs were infec...
متن کاملBone morphogenetic protein 4 induces efficient hematopoietic differentiation of rhesus monkey embryonic stem cells in vitro.
A cell culture system consisting of mouse S17 stromal cells supplemented with cytokines was developed for hematopoietic differentiation of rhesus monkey embryonic stem (ES) cells. The differentiated colonies that formed contained clusters of hematopoietic-like cells, as well as structures similar in appearance to embryonic blood islands. When this culture system was supplemented with bone morph...
متن کاملOsteogenic Differentiation of Mesenchymal Stem Cells Induced by Bone Morphogenetic Protein-4: Cross-talk among Different Signaling Pathways
*Ho, CY; *Chen, WM; *Chen, TH; +*Lee, OK +*Taipei Veterans General Hospital, Taiwan [email protected] Introduction: Bone morphogenetic protein-4 (BMP-4) possesses osteoinductive activities and is known to induce osteogenic differentiation in mesenchymal stem cells (MSCs). It has been reported that BMP signaling is associated with the PTEN/PI3 Kinase/AKT signaling pathway which subsequently al...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Arthritis Research & Therapy
سال: 2009
ISSN: 1478-6354
DOI: 10.1186/ar2822